@article{Mendoza-Azpur_Ortiz_Valdivia_De Souza_Collares_Meza_Shibli_2021, title={Cyanoacrylate tissue adhesive as a membrane in socket preservation: In vitro cytotoxicity and clinical evaluation}, volume={13}, url={https://journalofosseointegration.eu/jo/article/view/439}, DOI={10.23805/JO.2021.13.04.7}, abstractNote={<p class="p1"><strong>Aim </strong>The aim of the present study was to demonstrate the <em>in vitro</em> effect of cyanoacrylate-based tissue adhesive and the clinical evaluation of wound healing of freshly extracted sockets with adhesives applied on an exposed collagen membrane, for preservation of the alveolar ridge.</p> <p class="p1"><strong>Materials and methods</strong><span class="Apple-converted-space">  </span>Cytotoxicity was evaluated with sulphoradamine B assay with primary fibroblasts from gingival tissue. The <em>in vivo</em> analysis was carried out in 10 patients needing extraction of single-rooted teeth, who met the inclusion and exclusion criteria and signed the term of free and informed consent. Alveolar preservation was carried out with a mineralized cortical allograft bone substitute and a resorbable collagen membrane that was intentionally left exposed. On the exposed membrane, the cyanoacrylate-based tissue adhesive<span class="Apple-converted-space">  </span>(Periacryl® 90 HV) was applied as a barrier.<span class="Apple-converted-space">  </span>The biocompatibility of the adhesive was assessed by the Early-Wound Healing Index (EHI) and Early Wound Healing Score (EHS), of perialveolar tissues at time intervals of 12, 30 and 60 days post surgery.</p> <p class="p1"><strong>Results</strong> Reduced cell viability was observed for Periacryl® 90 HV when compared with cells without adhesive treatments. No postoperative complications were observed after the application of the adhesive on perialveolar tissues.</p> <p class="p1"><strong>Conclusion </strong>The use of the cyanoacrylate-based tissue adhesive resulted in reduced fibroblast viability <em>in vitro</em>, and adequate results of wound closure in the clinical evaluation of EHI and EHS scores.</p>}, number={4}, journal={Journal of Osseointegration}, author={Mendoza-Azpur, G. and Ortiz, J. and Valdivia, E. and De Souza, G. and Collares, F. and Meza, J. and Shibli, J.}, year={2021}, month={Oct.}, pages={220–226} }