Osteoproperties of polyethylene glycol hydrogel material


Submitted: 13 June 2017
Accepted: 13 June 2017
Published: 30 October 2014
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Authors

  • Diego Lops Department of Prosthodontics, University of Milan, School of Dentistry, Dental Clinic, S. Paolo Hospital, Milano, Italy.
  • Letizia Ferroni Department of Biomedical Sciences, University of Padua, Padua, Italy.
  • Chiara Gardin Department of Biomedical Sciences, University of Padua, Padua, Italy.
  • Sara Ricci Department of Neurosciences, University of Padua, Padua, Italy.
  • Riccardo Guazzo Department of Neurosciences, University of Padua, Padua, Italy.
  • Luca Sbricoli Department of Neurosciences, University of Padua, Padua, Italy.
  • Eugenio Romeo Department of Prosthodontics, University of Milan, School of Dentistry, Dental Clinic, S. Paolo Hospital, Milano, Italy.
  • José L. Calvo-Guirado Department of General Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
  • Eriberto Bressan Department of Neurosciences, University of Padua, Padua, Italy.
  • Barbara Zavan Department of Biomedical Sciences, University of Padua, Padua, Italy.
Aim The aim of the present study was to test the osteogenic potential of a synthetic hydrogel made of polyethylene glycol (PEG), loaded with adult mesenchymal stem cells, used as a biodegradable membrane for guided bone regeneration (GBR). Materials and methods Adult mesenchymal stem cells derived from adipose tissue (ADSCs) were isolated, characterized, and seeded on the hydrogel. After 15 days of culture, the scaffolds were analyzed with scanning electron microscopy (SEM) and real-time PCR to assess osteogenesis, and by means array CGH (Comparative Genomic Hybridization) to test their safety. Results The in vitro results confirmed that the ADSCs were able to attach to the hydrogel and differentiate towards the osteogenic phenotype. Furthermore, array CGH analysis detected no chromosomal abnormalities, confirming the safety of the 3D cultures. Conclusion The PEG hydrogel, loaded with adult mesenchymal stem cells, seems to have an osteogenic potential and therefore could be successfully used as a membrane in the treatment of bone defects.

Supporting Agencies


Lops, D., Ferroni, L., Gardin, C., Ricci, S., Guazzo, R., Sbricoli, L., Romeo, E., Calvo-Guirado, J. L., Bressan, E., & Zavan, B. (2014). Osteoproperties of polyethylene glycol hydrogel material. Journal of Osseointegration, 6(3), 61–65. https://doi.org/10.23805/jo.2014.06.03.04

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