https://doi.org/10.23805/jo.2011.03.01.03

Effect of porous titanium coating thickness on in vitro osteoblast phenotype expression

Vol. 3 No. 1 (2011)
Submitted: 8 June 2017
Accepted: 8 June 2017
Published: 30 March 2011
Bone, Cell culture, Cell-material interactions, Porous coated, Titanium
Abstract Views: 521
PDF: 538
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Authors

Antonio Canabarro
info@ariesdue.it
DDS, MSc, PhD Department of Periodontology, School of Dentistry of Rio de Janeiro State University, RJ, Brazil.
Grasiele E. Crippa
DDS, MSc, PhD, Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, SP, Brazil.
Mariana U. Shirozaki
DDS, Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, SP, Brazil.
Eduardo M. Sampaio
PE, MSc, PhD, Adhesion and Adherence Laboratory, Department of Mechanical Engineering of Rio de Janeiro State University, RJ, Brazil.
Louis-Philippe Lefebvre
PE, PhD, National Research Council Canada, Industrial Materials Institute, Boucherville, Canada.
Paulo T. De Oliveira
DDS, MSc, PhD, Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Brazil.
Marcio M. Beloti
DDS, MSc, PhD, Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Brazil.
Adalberto L. Rosa
DDS, MSc, PhD, Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Italy.
Aim This study aimed at determining the effect of different thickness of porous titanium (Ti) coating, 0.5, 1.0 and 1.5 mm thick (PC-0.5, PC-1.0 and PC-1.5), on osteoblast phenotype expression. Materials and methods Dense Ti discs coated with 0.5, 1.0 and 1.5 mm of porous Ti (PC-0.5, PC-1.0 and PC-1.5, respectively) were fabricated by powder metallurgy process with pore size typically between 50 and 400 μm and porosity of 60%. Osteoblastic cells obtained from human alveolar bone were cultured on dense Ti (D-Ti) and PC-Ti discs for periods of up to 17 days. Results Cultures grown on PC-Ti exhibited higher cell proliferation rate than on D-Ti. By comparing PC-Ti groups, it was observed statistical differences on culture grown only at day 10 (PC- 0.5<PC-1.0 and PC-1.5). Cells cultured on PC-1.5 presented the highest gene expression of type I collagen (COL), alkaline phosphatase (ALP), and osteocalcin (OC). The calcium content was significantly greater on PC-1.5 compared to all other groups. Conclusion These results indicate that PC-Ti favored osteoblastic cell proliferation. In addition, they increased gene expression of osteoblastic markers and higher content of mineralized matrix was observed on the thicker PC-Ti coating (PC-1.5). Therefore, further in vivo evaluations should be done in order to investigate whether this structure should be considered for clinical implant applications.

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How to Cite

Canabarro, A., Crippa, G. E., Shirozaki, M. U., Sampaio, E. M., Lefebvre, L.-P., De Oliveira, P. T., Beloti, M. M., & Rosa, A. L. (2011). Effect of porous titanium coating thickness on in vitro osteoblast phenotype expression. Journal of Osseointegration, 3(1), 17–23. https://doi.org/10.23805/jo.2011.03.01.03

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