Effect of porous titanium coating thickness on in vitro osteoblast phenotype expression
AbstractAim This study aimed at determining the effect of different thickness of porous titanium (Ti) coating, 0.5, 1.0 and 1.5 mm thick (PC-0.5, PC-1.0 and PC-1.5), on osteoblast phenotype expression. Materials and methods Dense Ti discs coated with 0.5, 1.0 and 1.5 mm of porous Ti (PC-0.5, PC-1.0 and PC-1.5, respectively) were fabricated by powder metallurgy process with pore size typically between 50 and 400 μm and porosity of 60%. Osteoblastic cells obtained from human alveolar bone were cultured on dense Ti (D-Ti) and PC-Ti discs for periods of up to 17 days. Results Cultures grown on PC-Ti exhibited higher cell proliferation rate than on D-Ti. By comparing PC-Ti groups, it was observed statistical differences on culture grown only at day 10 (PC- 0.5<PC-1.0 and PC-1.5). Cells cultured on PC-1.5 presented the highest gene expression of type I collagen (COL), alkaline phosphatase (ALP), and osteocalcin (OC). The calcium content was significantly greater on PC-1.5 compared to all other groups. Conclusion These results indicate that PC-Ti favored osteoblastic cell proliferation. In addition, they increased gene expression of osteoblastic markers and higher content of mineralized matrix was observed on the thicker PC-Ti coating (PC-1.5). Therefore, further in vivo evaluations should be done in order to investigate whether this structure should be considered for clinical implant applications.
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Copyright (c) 2011 Antonio Canabarro, Grasiele E. Crippa, Mariana U. Shirozaki, Eduardo M. Sampaio, Louis-Philippe Lefebvre, Paulo T. De Oliveira, Marcio M. Beloti, Adalberto L. Rosa
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