Enhancement of Osseointegration of the Hydroxyapatite implant by Low intensive Ultrasound wave (LIPUS) Irradiation
Aim The purpose of this study is to investigate the enhancement effect of osseointegration of low intensity ultrasound waving for reinforcement of bone- attachment of hydroxyapatite coating implant.
Materials and methods In this study, hydroxyapatite specimen were prepared, two kinds of experiments ( simulation test and animal experiment ) were performed.
Hydroxyapatite specimen were soaked in the simulated body fluid (SBF) as a simulation test, implanted rabbits iliac crest as in vivo test. After the irradiation of pulsed ultrasound wave for some planned time–periods, the surface of specimens were assessed and compared with non-ultrasound waving specimens ( control group ) by using Scanning Electron Microscope (SEM), Energy Dispersive Spectroscop (EDS) and X-ray diffraction.
Results SEM image and EDS showed that richer layer of bone-like hydroxyapatite covered specimens surfaces in ultrasound wave irradiation group as compared with control group. The measurement of mass of specimens also indicated the efficiency of ultrasound waves for hydroxyapatite formation. These results indicated low intensity ultrasound wave might promote the nucleation and crystallization of bone-like apatite on hydroxyapatite surfaces.
Conclusion This study suggested that the clinical application of ultrasound waving has a great potential for enhancement of osseointegration of hydroxyapatite dental implant through the activation of bone bonding mechanism on material surface.
2. Brånemark P I. Osseointegration and its experimental background. J. Pros Dentistry. 1983; 50: 399-410.
3. Smeets R, Stadlinger B, Schwarz F, Beck-Broichsitter B, Jung O, Precht C, Kloss F,Alexander Gröbe A, Heiland M, Ebker T. Impact of Dental Implant Surface Modifications on Osseointegration BioMed Res Inter. 2016 ;1-16.
4. Thomas KA, Kay JF, Cook SD, Jarcho M. The effect of surface macrotexture and hydroxyapatite coating on the mechanical strength and histologic profiles of titanium implant materials. J.Biomed.Mater.Res. 1987; 21: 1395-1406
5. De Andrade MD, Sader MS, Filgueiras MRT, Ogasawara T. Microstructure of ceramic coating on titanium surface as a result of hydrothermal treatment. J. Mater Sci : Mater Med. 2000 ; 11 : 751-55
6. Heckman JD, Ryaby JP, McCabe J, Frey JJ, Kilcoyne RF. Acceleration of tibial fracture-healing by non-invasive, low intensity pulsed ultrasound. J.Bone.Joint.Surg.(A) 1994 ; 76 : 26-34
7. Kristiansen TK, Ryaby JP, McCabe J, Frey JJ, Roe LR. Accelerated healing of distal radial fractures with the use of specific, low-intensity ultrasound. A multicenter, prospective, randomaized, double-blind, placebo-controlled study. J.Bone.Joint.Surg.(A) 1997 ; 79 : 961-73
8. Tanzer M, Kantor S and Bobyn JD. Enhancement of bone growth into porous intramedullary implants using non-invasive low intensity ultrasound. J Orthop Res 2001;19 : 195-199
9. Rego EB, Takata T, Tanne K and Tanaka E. Current status of low intensity pulsed ultrasound for dental purposes. Open Dent J. 2012; 6: 220-225
10. Ichitsubo T, Matsubara E, Kai S, Hirano M. Ultrasound-induced crystallization around the glass transition temperature for Pd40Ni40P20 metallic glass. Acta.Mate. 2004 ; 52 : 423-429
11. Ichitsubo T, Matsubara E, Anazawa K, Nishiyama N. Crystallization accelerated by ultrasound in Pd-based metallic glasses. J. Alloy.Compounds. 2007 ; 434-435 : 194-195
12. Kobayashi M, Noda K, Tatematsu N. Preliminary in vitro study on Enhancement of Bone-like Hydoxyapatite formation on bioactive titanium alloy by low-intensity pulsed ultrasound waving for early bone bonding. J. Biomech. Sci and Eng. 2010 ; 4 : 449-460
13. Kobayashi M Study on enhancement of Osseointegration of the bio-active titanium implant by low intensive Ultrasound wave. (Part1: Simulated body fluid soaking test). Int. J. Biomed. Eng. Sci (IJBES) 2017 ; 4 : 1-10
14. Kobayashi M, Shiraishi M, Kinoshita M Study on enhancement of Osseointegration of the bio-active titanium implant by low intensive Ultrasound wave. (Part2: In vivo Experiment). Int. J. Biomed. Eng. Sci (IJBES) 2018; 1 : 1-10
15. Kokubo T, Takamada H. How useful is SBF in predicting in vivo bone bioactivity?
Biomaterials 2006 ; 27: 2907-15
16. Kokubo T, Miyaji F, Kim HM, Nakamura T. Spontaneous formation of bonelike apatite layer on chemically treated titanium metals. J. Am. Cerami. Soc. 1996 ; 79 : 1127-1129
17. Kokubo T, Yamaguchi S. Novel Bioactive Titanate Layers Formed on Ti Metal and Its Alloy by Chemical Treatments. Materials. 2010; 3: 48-63
18. Yamaguchi S, Akeda K,Murata K,Takegami N,Goto M, Kokubo T. Chemical and Heat Treatments for Inducing Bone-Bonding Ability of Ti-6Al-4V Pedicle Screw. Key Eng. Mate. 2015 ; 631: 225-230
19. Mukai H, Kobayashi M The Effect of Low-intensive Pulsed Ultrasound Waving on Hydroxyapatite in Simulated Body Fluid Proceed. of IEEE 11th Inter. Confer. on Bioinf. Bioeng (BIBE) IEEE Computer Society 2011; Taipei : 125-8
20. Sato T, Shimizu Y, Odashima K, Sano Y, Yamamoto A, Mukai T, Ikeo N, Takahashi T, Kumamoto H. In vitro and in vivo analysis of the biodegradable behavior of a magnesium alloy for biomedical applications Dent. Mate J. 2018 ;1: 1-11
21. ZhaoW, Michalik D, Ferguson S, Hofstetter W, Lemaître J, von Rechenberg, Bowen P. Rapid evaluation of bioactive Ti-based surfaces using an in vitro titration method. Nature commun. 2019 ; 10 : 2062
22. Hurle D. T. J. Handbook of crystal growth. North Holland;1993,
23. Teraoka Y, Saito A, Okawa S. Ice crystal growth in supercooled solution Int. J .Refrigeration 2002 ; 25 : 218-225
24. Tanaka A, Sawada K. The Effect of the Pulse ultrasonic waves in the dendritic crystal growth. Bussei.Kenkyu. 1989 ; 52 : 430-434 ( in Japanese )
25. Ichitsubo T, Matsubara E, Anazawa K, Nishiyama N, Naito M, Hirotsu Y. Low-temperature crystallization caused by ultrasound in Pd42.5Ni7.5Cu30P20 and Pd40Ni40P20 bulk metallic glasses. Mate.Sci.Eng. 2006 ; A442 : 273-277
26. Abdulhameed EA, Enezei HH, Omar M, Komori , Sugita Y, Hegazy FA, Ari S, Maeda H, Alam MK The Effect of Low Intensity Pulsed Ultrasound Therapy on Osseointegration and Marginal Bone Loss Around Dental Implants. J. Hard Tissue Bio 2017; 26 :323-330
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