Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices

  • A. Pozzuoli | info@ariesdue.it Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Padua, Italy, Italy.
  • C. Gardin Department of Biomedical Sciences, University of Padua, Padua, Italy, Italy.
  • R. Aldegheri Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Padua, Italy, Italy.
  • E. Bressan Department of Neurosciences, University of Padua, Padua, Italy, Italy.
  • M. Isola
  • J. L. Calvo-Guirado Department of General Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain, Italy.
  • C. Biz Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Padua, Italy, Italy.
  • P. Arrigoni Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Padua, Italy, Italy.
  • L. Feroni Department of Biomedical Sciences, University of Padua, Padua, Italy, Italy.
  • B. Zavan Department of Biomedical Sciences, University of Padua, Padua, Italy, .

Abstract

Aim Tissue engineering is a rapidly expanding field with regard to the use of biomaterials and stem cells in the orthopedic surgery. Many experimental studies have been done to understand the best characteristics of cells, materials and laboratory methods for safe clinical applications. The aim of this study was to compare the ability of 2 different human demineralized bone matrices (DBMs), the one enriched and the other not enriched with hyaluronic acid, to stimulate in vitro the proliferation and the osteogenic differentiation of human adipose-derived stem cells (ADSCs) seeded onto an osteoconductive scaffold.

Materials and Methods ADSCs were isolated, by enzymatic digestion, from abdominal adipose tissue of 5 patients undergoing cosmetic lipoaspiration surgery. ADSCs were then seeded onto a 3D scaffold in the presence of the two different osteoinductive matrices of human demineralized bone and evaluated for proliferation and osteogenic differentiation. The safety of the methods was verified using array-Comparative Genomic Hybridization (array-CGH). 

Results ADSCs were able to differentiate in osteogenic sense. Both DBMs showed the ability to induce osteogenic differentiation of the cells. 

Conclusion array-CGH showed no changes at genome level, thus confirming the safety of materials and methods.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

References

Giannoudis PV, Einhotn TA, Marsh D. Fracture healing: the diamond concept. Injury 2007;38S4:S3-S6.

Logeart-Avramoglou D, Anagnostou F, Bizios R, Petite H. Engineering of bone: challenges and obstacles. J Cell Mol Med 2005;9:72-84.

Samartzis D, Shen FH, Goldberg EJ, An HS. Is autograft the gold standard in achieving radiographic fusion in one-level anterior cervical discectomy and fusion with rigid anterior plate fixation? Spine 2005;30:1756-1761.

Bauer TW, Muschler GF. Bone graft materials: an overview of the basic science. Clin Orthop Relat Res 2000;371:10-27.

Damien CJ, Parsons JR. Bone graft and bone graft substitutes: A review of current technology and applications. J Appl Biomater 1991;2:187-208.

Greenwald AS, Boden SD, Goldberg VM, Khan Y, Laurencin CT, Rosier RN. Bone-graft substitutes: facts, fictions, and applications. J Bone Joint Surg Am 2001;83A Suppl2:98-103.

Finkemeier CG. Bone-grafting and bone graft substitutes. J Bone Joint Surg Am 2002;84A:454-464.

Putzier M, Strube P, Funk JF, Gross C, Monig HJ, Perka C, Pruss A. Allogenic versus autologous cancellous bone in lumbar segmental spondylodesis: a randomized prospective study. Eur Spine J 2009;18:687-695.

Dimitriou R, Mataliotakis GI, Angoules AG, Kanakaris NK, Giannoudis PV. Complications following autologous bone graft harvesting from the iliac crest and using the RIA: a systematic review. Injury 2011;42 Suppl2:S3-15.

Nocini PF, Bedogni A, Valsecchi S, Trevisiol L, Ferrari F, Fior A, Saia G. Fractures of the iliac crest following anterior and posterior bone graft harvesting. Review of the literature and case presentation. Minerva Stomatol 2003;52:441-448.

Weikel AM, Habal MB. Meralgia paresthetica: a complication of iliac bone procurement. Plast Reconstr Surg 1977;60:572-574.

Shi Y , Niedzinski JR, Samaniego A, Bogdansky S, Atkinson BL. Adipose-derived stem cells combined with a demineralized cancellous bone substrate for bone regeneration. Tissue Eng Part A 2012;18:1313-1321.

Kim HP, Ji YH, Rhee SC, Dhong ES, Park SH, Yoon ES. Enhancement of bone regeneration using osteogenic-induced adipose-derived stem cells combined with demineralized bone matrix in a rat critically-sized calvarial defect model. Curr Stem Cell Res Ther 2012;7:165-172.

Ghosh SK, Nandi SK, Kundu B, Datta S, De DK, Roy SK, Basu D. In vivo response of porous hydroxyapatite and β-tricalcium phosphate prepared by aqueous solution combustion method and comparison with bioglass scaffolds. J Biomed Mater Res B Appl Biomater 2008;86:217-227.

Daculsi G, LeGeros RZ, Heughebeart M, Barbieux I. Formation of carbonate apatite crystals after implantation of calcium phosphate ceramics. Calcif Tissue Int 1990;46:20-27.

Daculsi G. Biphasic calcium phosphate concept applied to artificial bone, implant coating and injectable bone substitute. Biomaterials 1988;19:1473-1478.

Gauthier O, Bouler JM, Weiss P, Bosco J, Daculsi G, Aguado E. Kinetic study of bone ingrowth and ceramic resorption associated with the implantation of different injectable calcium phosphate bone substitutes. J Biomed Mater Res 1999;47:28-35.

Kuhne JH, Barti R, Frisch B, Hammer C, Jansson V, Zimmer M. Bone formation in coralline hydroxyapatite. Effects of pore size studied in rabbits. Acta Orthop Scand 1994;65:246-252.

Wykrota LL, Wykrott FHL, Garrido CA. Long-term bone regeneration in large human defects using calcium-phosphate particulate. In: Davies JE ed. Bone Engineering. Em square incorporated Publ. Toronto, Canada, 2000;p516-565.

Nandi SK, Roy S, Mukherjee P, Kundu B, De DK, Basu D. Orthopaedic applications of bone graft & graft substitutes: a review. Indian J Med Re 2010;132:15-30.

Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 2001;7:211-228.

Strem BM, Hicok KC, Zhu M, Wulur I, Alfonso Z, Schreiber RE, Fraser JK, Hedrick MH. Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med 2005;54:132-141.

Zhu X, Shi W, Tai W, Liu F. The comparition of biological characteristics and multilineage differentiation of bone marrow and adipose derived Mesenchymal stem cells. Cell Tissue Res 2012;350:277-287.

Housman TS, Lawrence N, Mellen BG, George MN, Filippo JS, Cerveny KA, DeMarco M, Feldman SR, Fleischer AB. The safety of liposuction: results of a national survey. Dermatol Surg 2002;28:971-978.

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop DJ, Horowitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006;8:315-317.

Pardal R, Clarke MF, Morris Sj: Applying the principles of stem-cell biology to cancer. Nat Rev Cancer 2003;3:895-902.

Marx J: Cancer research. Mutant stem cells may seed cancer. Science 2003;301:1308-1310.

Krampera M, Pasini A, Rigo A, Scupoli MT, Tecchio C, Malpeli G, Scarpa A, Dazzi F, Pizzolo G, Vinante F. HB-EGF/HER-1 signaling in bone marrow mesenchymal stem cells: inducing cell expansion and reversibly preventing multilineage differentiation. Blood 2005;106:59-66.

Shien K, Toyooka S, Yamamoto H, Soh J, Jida M, Thu KL, Hashida S, Maki Y, Ichiara E, Asano H, Tsukuda K, Takigawa N, Kiura K, Gazdar AF, Lam WL, Miyoshi S. Acquired resistence to EGFR inhibitors is associated with a manifestation of stem-like properties in cancer cells. Cancer Res 2013;73:3051-3061.

Clevers H: Wnt/β-catenin signaling in development and disease. Cell 2006;127:469-480.

MacDonald BT, Tamai K, He X: Wnt/β-catenin signaling: components, mechanisms, and diseases. Dev Cell 2009;17:9-26.

Clevers H, Nusse R: Wnt/β-catenin signaling and disease. Cell 2012; 149: 1192-1205.

Izadpanah R, Kaushal D, Kriedt C, Tsien F, Patel B, Dufour J,Bunnell BA. Long-term in vitro expansion alters the biology of adult mesenchymal stem cells. Cancer Res 2008;68:4229-4238.

Rosland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, Immervoll H, Mysliwietz J, Tonn JC, Goldbrunner R, Lonning PE, Bjerkvig R, Schichor C. Long-term cultures of bone marrow-derived human mesenchymal stem cells frequently undergo spontaneous malignant transformation. Cancer Res 2009;69:5331-5339.

Shinawi M, Cheung SW. The array CGH and its clinical applications. Drug Discov Today 2008;13:760-770.

Thorwarth M, Schlegel KA, Wehrhan F, Srour S, Schultze-Mosqau S. Acceleration of de novo bone formation following application of autogenous bone to particulated anorganic bovine material in vivo. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:309-316.

Aguiari P, Leo S, Zavan B, Vindigni V, Rimessi A, Bianchi K, Franzin C, Cortivo R, Rossato M, Vettor R, Abatangelo G, Pozzan T, Pinton P, Rizzuto R. High glucose induces adipogenic differentiation of muscle-derived stem cells. Proc Natl Acad Sci U S A 2008;105:1226-1231.

Strober W. Trypan blue exclusion test of cell viability. Curr Protoc Immunol 2001;Appendix 3:Appendix 3B.

Denizot F, Lang R. Rapid colorimetric assay for cell-growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 1986,89:271-277.

Asagiri M, Takayangi H. The molecular understanding of osteoclast differentiation. Bone 2007;40:251-264.

Cohen MM Jr. The new bone biology: pathology, molecular, and clinical correlates. Am J Med Genet 2006;140:2646-2706.

Landis WJ. The strength of a calcified tissue depends in part on the molecular structure and organization of its constituent mineral crystals in their organic matrix. Bone 1995;16:533-544.

Kouropis D, Baboolal TG, Jones E, Giannoudis PV. Native multipotential stromal cell colonization and graft expander potential of a bovine natural bone scaffold. J Orthop Res 2013;31:1950-1958.

Gardin C, Bressan E, Ferroni L, Nalesso E, Vindigni V, Stellini E, Pinton P, Sivolella S, Zavan B. In vitro concurrent endothelial and osteogenic commitment of adipose-derived stem cells and their genomical analyses through comparative genomic hybridization array: novel strategies to increase the successful engraftment of tissue-engineered bone grafts. Stem Cells Dev 2011;21:767-777.

Published
2015-03-30
Info
Issue
Section
Articles
Keywords:
Adipose-derived stem cells, Array-CGH, Demineralized bone matrices, Osteogenesis, Tissue engineering
##article.stats##
  • Abstract views: 341

  • PDF: 217
How to Cite
Pozzuoli, A., Gardin, C., Aldegheri, R., Bressan, E., Isola, M., Calvo-Guirado, J. L., Biz, C., Arrigoni, P., Feroni, L., & Zavan, B. (2015). Genetical stability and osteogenic ability of mesenchimal stem cells on demineralized bone matrices. Journal of Osseointegration, 7(1), 2-7. https://doi.org/10.23805/jo.2015.07.01.01