Journal of Osseointegration

Evaluation of the Efficiency of Different Irrigation Materials to Remove the Bioceramic Sealer in Endodontic Retreatment

W. S. Haichal — 2025-09-17

Aim

This research aimed to assess the effectiveness of irrigants (phosphoric acid, EDTA, and Triton) in eliminating the bioceramic sealer and smear layer from dentinal tubules during retreatment.

Materials and Methods

Twenty-seven single roots (n=9) were collected. All samples were instrumented using the WaveOne Gold file system (Dentsply Sirona Pty Ltd, Charlotte, NC, USA). Irrigated with 5% NaOCl and 17% EDTA. Subsequently, the obturation used cold and warm techniques with Ceraseal bioceramic sealer (Meta Biomed, Republic of Korea). Pre- and post-endodontic therapy periapical X-rays were done. then incubated at 37°C in 100% humidity for two weeks. Reinstrumentation was performed using the ProTaper Gold system (Dentsply Sirona Pty Ltd, Charlotte, NC, USA). The samples were randomly divided into three groups. Group (A): Phosphoric acid 37% Group (B): EDTA 17% with ultrasonic activation Group (C): TRITON using ultrasonic activation, all samples were longitudinally sectioned using a low-speed saw (Isomet Buehler Co., USA); hereafter, chosen portions were affixed to matrices and gold-sputtered using a sputtering apparatus. The samples were examined with a scanning electron microscope (Jsm-6060LV, Jeol Ltd., Tokyo, Japan) for evaluation.

Results

The results of the bioceramic removal showed that the EDTA was statistically more effective than phosphoric acid and Triton in removing the bioceramic sealer (p<0.05), except for the phosphoric acid with single cone obturation reported better efficiency and Triton with single cone technique in the coronal part only, then reported reduced effectiveness significantly in the middle and apical parts (p>0.05).

Conclusion

This research concludes that EDTA is more successful than phosphoric acid and Triton in eliminating the bioceramic sealer and smear layer from root canals. None of the tested supplementary cleaning materials or techniques completely removed the residual filling material.

Randomized clinical trial on clinical performance of sectional non – invasive laminate veneers using the modified US Public Health System criteria

P. Papanagiotou — 2025-06-12

Purpose To compare the clinical performance of Sectional non – invasive laminate veneers (SNIVs) in patients with tooth diastemas in the frontal area.

Methods Ten patients with tooth diastema in the frontal area were included in this study. Each tooth diastema was treated with one feldspathic and one lithium disilicate sectional non-invasive laminate veneer. The veneers were evaluated by two investigators at baseline, immediately after cementation, at four and at twelve months of function according to the modified United States Public Health Service (USPHS) criteria. The parameters under evaluation included: anatomical form, marginal discoloration, marginal integrity, restoration color stability, secondary caries and surface texture.

Results Based on the clinical evaluation by the investigators, both groups had alterations in their anatomical form, developed marginal discoloration and loss of marginal integrity. On the other hand, the color of the restorations and the surface texture had no significant changes and no secondary caries were detected.

Conclusions Both groups of SNIVs had an acceptable clinical performance within 12 months of function in the oral cavity.

Evaluation of Color Stability of 3D Printing Resins After Exposure to Various Colorants: an in vitro study

G. Verniani — 2025-06-17

Purpose. This study investigates the color stability of four types of 3D printing resins: TempPrint (TP) (GC), Varseo Smile Teeth (VST) (Bego), TrinQ (TQ)(Bego), and V-Print(VP) (Voco) when exposed to various colorants over a 30-day period.

Methods. A total of 200 disk-shaped specimens (10 mm × 2 mm) were printed using a DLP printer and exposed to four colorants: wine, Coca-Cola, tea, coffee and distilled water. Color changes were measured at 7, 15, and 30 days and total color differences (ΔE) were calculated.

Results. The results revealed significant discoloration across all resins, with wine and coffee inducing the most pronounced color changes. TP (GC) demonstrated the least discoloration in all solutions, particularly in water and Coca-Cola. VST(Bego) showed the highest degree of discoloration, especially in wine and coffee, with ΔE values reaching 20.54 after 30 days of immersion in coffee. TQ (Bego) and VP (Voco) exhibited intermediate levels of discoloration, with coffee causing the most severe color changes. Statistical analysis indicated significant differences in color stability between the resins, with wine and coffee causing more discoloration.

Clinical significance. These findings suggest that while all resins undergo color change when exposed to common beverages, the extent of discoloration varies based on resin composition, highlighting the need for material selection based on expected environmental exposure in practical applications.

A novel therapeutic strategy for periodontal regeneration of non-contained intrabony defects using autologous micrografts from the palatal mucosa

M. Aimetti — 2025-06-09

Aims: Due to their accessibility and biological features, mesenchymal stem cells (MSCs) derived from the hard palate mucosa (PMSCs) hold significant promise for periodontal re-generation. This case series investigates the clinical and radiographic efficacy of autologous micrografts (AMGs) enriched in PMSCs for the treatment of non-contained defects in patients with severe periodontitis.

Materials and methods: Five patients presenting with at least one predominantly 1- or 2-wall intra-bony defect requiring periodontal regenerative surgery were consecutively enrolled. A small connective tissue sample was harvested from the palate, mechanically dissociated chair-side, and filtered to obtain AMGs enriched in PMSCs. The selected intrabony defects were filled with a resorbable scaffold seeded with a suspension containing AMGs.

Results: At the 6-month follow-up, a mean clinical attachment gain of 4.8 ± 1.8 mm was observed, along with a residual mean probing depth of 4.2 ± 0.8 mm and a radiographic bone fill of 3.6 ± 4.3 mm Characterization of AMGs was performed in two patients, demonstrating progenitor cells expressing MSC-specific surface markers.

Conclusions: These preliminary findings suggest that AMGs derived from the palatal mucosa may offer a promising approach for the regenerative treatment of intrabony defects with unfavorable architecture.